The expression of cytochrome P450 1A1 of endothelial cells induced by benzo(a)pyrene / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 362-364, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-340036
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of benzo(a)pyrene (BaP) on the expression and the activities of cytochrome P450 1A1 (CYP1A1) of porcine aortic endothelial cells.</p><p><b>METHODS</b>Porcine aortic endothelial cells were cultured in vitro, and treated with different concentrations of BaP (0, 0.5, 1.0, 5.0, 10.0 micro mol/L) for 24 hours, CYP1A1 expression was determined by Western blot and immunohistochemistry. At the same time, the ethoxyresorufin-o-deethylase (EROD) activities were measured by spectrofluorometer.</p><p><b>RESULTS</b>By Western blot, the expression of CYP1A1 of control cells was not found, but the expression of CYP1A1 of cells treated with BaP was found; By immunohistochemistry, only part of endothelial cells treated with BaP had positive expression of CYP1A1. The peak activities of EROD induced by BaP was at the concentration of 0.5 - 1.0 micro mol/L.</p><p><b>CONCLUSION</b>BaP could induce part of endothelial cells to synthesize CYP1A1. BaP of 0.5 - 1.0 micro mol/L could induce peak activities of EROD.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Swine
/
Benzo(a)pyrene
/
Endothelium, Vascular
/
Immunohistochemistry
/
Cells, Cultured
/
Blotting, Western
/
Cytochrome P-450 CYP1A1
/
Cell Biology
/
Dose-Response Relationship, Drug
/
Toxicity
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2003
Type:
Article
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