Study on three kinds of gasoline oxygenates-induced DNA damage in mice fibroblasts / 中华劳动卫生职业病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study DNA damage of three kinds of gasoline oxygenates.</p><p><b>METHOD</b>Single cell gel electrophoresis assay(Comet assay) was used to detect the damage effects of three gasoline oxygenates[methyl tertiary butyl ether(MTBE), ethanol anhydrous(EA) and dimethyl carbonate(DMC)] on DNA in L-929 mice fibroblasts.</p><p><b>RESULTS</b>In certain concentation(37.500-150.000 mg/ml), MTBE could directly cause DNA damage of L-929 mice fibroblasts. There was obvious dose-effect relationship, i.e. when the concentration of MTBE was increased from 9.375 to 150.000 mg/ml, the comet rate also increased from 4% to 85%, and the length of comet tail changed correspondingly. The results of EA and DMC were negative.</p><p><b>CONCLUSION</b>Under the condition of this experiment(150.000 mg/ml), MTBE could directly cause DNA damage while the effect of EA and DMC on DNA damage was not found.</p>