Study on drug-resistance of Mycobacterium tuberculosis isolated from coal workers with silico-tuberculosis / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 100-102, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-340115
ABSTRACT
<p><b>OBJECTIVE</b>To study the gene mutation and streptomycin, isoniazid or rifampicin resistance of Mycobacterium isolated from silico-tuberculosis patient's sputum so as to find a more effective therapy for this disease.</p><p><b>METHODS</b>Mycobacteria tuberculosis were separated from 96 coal worker with silico-tuberculosis firstly. Then rpsL, KatG and rpoB fragments of genome were copied with PCR and compared their SSCP profiles with standard strains.</p><p><b>RESULTS</b>67 strains of streptomycin, isoniazid or rifampicin resistant Mycobacteria tuberculosis were found in routine drug resistance test, with the percentages of 80.5% (54/67), 58.2% (39/67) respectively. PCR-SSCP showed that out of 67 drug-resistant strains, 66(98.5%) of rpsL, 47(70.1%) of rpoB and 42(62.7%) of KatG appeared abnormal.</p><p><b>CONCLUSION</b>Most of the resistant strains appeared gene mutation. The mution rates were higher than the results from routine drug resistance test.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Silicotuberculosis
/
Sputum
/
Polymerase Chain Reaction
/
Coal
/
Polymorphism, Single-Stranded Conformational
/
Drug Resistance, Bacterial
/
Genetics
/
Microbiology
/
Mutation
/
Mycobacterium tuberculosis
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2002
Type:
Article
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