Effect of fractalkine on proliferation of pulmonary artery smooth muscle cells / 中国应用生理学杂志

ABSTRACT

<p><b>AIM</b>To investigate the effect of fractalkine on cell proliferation of cultured rat pulmonary artery smooth muscle cells (PASMCs) in vitro.</p><p><b>METHODS</b>Rat PASMCs were cultured in vitro, and treated with different concentrations (10(-10), 10(-9), 10(-8) mol/L) of fractalkine for 12 h, 24 h and 48 h. The cell proliferation was quantified by MTT assay. The cell cycle of PASMCs was measured by flow cytometric(FCM) analysis.</p><p><b>RESULTS</b>MTT assay showed that fractalkine promoted significantly the proliferation of PASMCs, and the effect was concentration-dependent. FCM analysis indicated that fractalkine increased the percentage of S phase and proliferative index (PI). The percentage of S phase and PI of PASMCs were increased after treated with fractalkine for 12 hours, which reached a maximal level at 24 hours.</p><p><b>CONCLUSION</b>Fractalkine promotes rat PASMCs proliferation in a concentration-dependent manner.</p>