Effect of progesterone on MMP-3 expression in neonatal rat brain after hypoxic-ischemia / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 370-373, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-340147
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of progesterone on matrix metalloproteinase-3 (MMP-3) expression in neonatal rat brain after hypoxic-ischemia.</p><p><b>METHODS</b>Followed the hypoxic-ischemia of neonatal rat brain, Evans blue (EB) staining and transmission electron microscopy were used to detect the blood-brain barrier pathological changes on permeability. MMP-3 protein expression in cerebral cortex was measured with Western blot.</p><p><b>RESULTS</b>Transmission electron microscopy results showed that the blood brain barrier in hypoxic-ischemic group changed significantly compare to progesterone group. EB staining results suggested that the blood-brain barrier permeability of hypoxic-ischemic group was significantly increased compared to sham-operated group (P < 0.01). The blood-brain barrier permeability in progesterone group was also decreased in comparison to that of hypoxic-ischemic group (P < 0.05). Western blot image analysis results indicated that MMP-3 protein expression in the hypoxic-ischemic group increased significantly than that in sham-operated group (P < 0.01), and the progesterone group was decreased significantly than that in hypoxic-ischemic group (P < 0.05).</p><p><b>CONCLUSION</b>Progesterone may reduce the blood-brain barrier damage by reducing MMP-3 expression. This might be one of the protective mechanisms in the hypoxic-ischemic brain injury.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Progesterone
/
Blood-Brain Barrier
/
Rats, Sprague-Dawley
/
Matrix Metalloproteinase 3
/
Hypoxia-Ischemia, Brain
/
Animals, Newborn
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2010
Type:
Article
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