The subgroup change of nitric oxide synthase in earlier period with cerebral ischemia/reperfusion in rats / 中国应用生理学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To observe the expression of endothelial nitric oxide synthase (eNOS)and nervous nitric oxide synthase (nNOS) in rats during cerebral ischemia/reperfusion (CI/R) and study if change will be happen in subgroup between eNOS and nNOS during earlier period of CI/R.</p><p><b>METHODS</b>A total of 60 Wistar rats weighting 200-280 g, supplied by Animal Center of China Medical University, were divided into 6 groups (n=10) (sham operation group; ischemia 1 h, 2 h group; reperfusion 0.5 h, 1 h, 2 h group). Female and male was half-and-half. Cerebral ischemia/reperfusion injury was induced by a 2-hour suture occlusion of the unilateral middle cerebral artery, immediately after suture withdrawal to allow reperfusion, eNOS and nNOS expressions were examined by the method of immunohistochemistry.</p><p><b>RESULTS</b>eNOS expressions increased in 1-hour during ischemia, keeping up with decreasing until reperfusion 2-hour. While nNOS expressions increased in 2-hour between ischemia and reperfusion.</p><p><b>CONCLUSION</b>Changes of expression between eNOS and nNOS in rats during cerebral ischemia/reperfusion are different. This may be related with ischemia and reperfusion injury.</p>