Hypoxia enhances the proliferation of skeletal myoblasts and possible mechanism / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 133-136, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-340215
ABSTRACT
<p><b>OBJECTIVE</b>To explore the role of hypoxia in skeletal myoblasts proliferation and related mechanism.</p><p><b>METHODS</b>The numbers and proliferous indexes of skeletal myoblasts were detected by flow cytometer under 20%, 3%, and 10% oxygen concentration. Hypoxia inducing factor 1alpha (HIF-1alpha) mRNA expression was determined by reverse transcriptase-polymerase chain reaction (RT-PCR). HIF-1alpha proteinum in endochylema and intranucelus were respectively detected by Western blot.</p><p><b>RESULTS</b>The numbers and proliferous indexes were higher (P < 0.05) in hypoxia group than those of control group. The expression HIF-1alpha mRNA had no difference in hypoxia and in normal groups. The level of HIF-1alpha proteinum in endochylema under normoxia was more than that in intranucelus and it was opposite under hypoxia.</p><p><b>CONCLUSION</b>Hypoxia can promote the proliferation of skeletal myoblasts. The possible mechanism of hypoxia promoting the proliferation of skeletal myoblasts might be that low oxygen concentration regulates HIF-la nuclear translocation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxygen
/
RNA, Messenger
/
Cell Hypoxia
/
Cells, Cultured
/
Rats, Wistar
/
Cell Biology
/
Myoblasts, Skeletal
/
Cell Proliferation
/
Hypoxia-Inducible Factor 1, alpha Subunit
/
Genetics
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2010
Type:
Article
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