Primary cell culture and identification methods of rat pulmonary arterial smooth muscle cells / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 125-128, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-340217
ABSTRACT
<p><b>OBJECTIVE</b>Set up a method to isolate and identify the small pulmonary arterial smooth muscle cells (PASMCs) in vitro.</p><p><b>METHODS</b>In sterile conditions, separated the male SD rat pulmonary artery, digested by collagenase I and cultured primary PASMCs. Measured cell viability; observed by phase contrast microscope; identified by immunocytochemistry and immunofluorescence staining as a label for smooth muscle alpha-actin.</p><p><b>RESULTS</b>PASMCs were identified by morphology and immunocytochemistry, immunofluorescence staining, with the cell viability is over 96.5%. The primary culture could be subcultured after 4-7 days and successfully passaged without change in morphology and growth characteristic.</p><p><b>CONCLUSION</b>This technique has advantage of the method is simple, short cultivate, good reproducibility, the primary cultured PASMCs quantity and the rapid growth.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Arterioles
/
Pulmonary Artery
/
Cell Separation
/
Rats, Sprague-Dawley
/
Cell Biology
/
Myocytes, Smooth Muscle
/
Primary Cell Culture
/
Lung
/
Methods
/
Muscle, Smooth, Vascular
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2010
Type:
Article
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