Mechanism of peripheral blood mononuclear cell invasion by HBV on artificial immunization in newborns / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1380-1382, 2002.
Article
in English
| WPRIM
| ID: wpr-340325
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect and mechanism of the peripheral blood mononuclear cell (PBMC) invasion by HBV on artificial immunization in newborns.</p><p><b>METHODS</b>Fifty-two newborns of HBsAg positive mothers were immunized with HBIG (hepatitis B immunoglobulin) and HBVac (hepatitis B vaccine) and were followed up for 7 months. The newborns' HBV-DNA in serum and in the PBMCs was detected with nested-PCR; anti-HBs was tested with solid phase radioimmunoassay (SP-RIA). PBMCs isolated from newborn peripheral blood were incubated in the presence of PHA or purified HBsAg. Interleukin-2 (IL-2) level in culture supernatants of activated cells was detected by ELISA.</p><p><b>RESULTS</b>The failure rate of immunization was higher in infants with positive HBV-DNA in PBMCs than those with negative HBV-DNA (P < 0.05); IL-2 level in PBMC culture supernatants was lower in former than in the latter and in normal controls (P < 0.05). The level of IL-2 in the immunization failure newborns was lower than that in the successfully immunized newborns and in normal controls (P < 0.05).</p><p><b>CONCLUSIONS</b>Intrauterine invasion of PBMCs by HBV is one of the important reasons for immunization failure in newborns. IL-2 production is closely related to the invasion of PBMCs by HBV, which may contribute to the failure of artificial immunization in newborns.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Virology
/
Blood
/
DNA, Viral
/
Immunoglobulins
/
Leukocytes, Mononuclear
/
Hepatitis B virus
/
Immunization
/
Interleukin-2
/
Hepatitis B Vaccines
Limits:
Humans
/
Infant, Newborn
Language:
English
Journal:
Chinese Medical Journal
Year:
2002
Type:
Article
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