Tissue tropism of the TTV in experimentally infected rhesus monkeys / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1088-1090, 2002.
Article
in English
| WPRIM
| ID: wpr-340379
ABSTRACT
<p><b>OBJECTIVE</b>To determine whether the transfusion-transmitted virus (TTV) is hepatotropic.</p><p><b>METHODS</b>Total DNA was extracted from various tissues of 5 experimentally infected Rhesus monkeys during the viremic period. A dot hybridization was done with viral double stranded DNA probes or single antisense probes.</p><p><b>RESULTS</b>The double-stranded probe was hybridized with DNA from the liver, bone marrow, spleen,stomach, small intestine and colon. The single-stranded antisense probe was hybridized with DNA from the liver, small intestine and bone marrow of all 5 monkeys, but not with that from other tissues.</p><p><b>CONCLUSIONS</b>As the viral genome was of negative polarity, the plus-stranded fragment identified in our study might be a replicative intermediate, and was only demonstrated in the liver, small intestine, and bone marrow by dot blot hybridization with single-stranded antisense probes. It is suggested that the TTV replicates in the liver, bone marrow and small intestine, and TTV might be hepatotropic.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Virology
/
Virus Replication
/
Bone Marrow
/
DNA, Viral
/
Torque teno virus
/
DNA Virus Infections
/
Genetics
/
Intestine, Small
/
Liver
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2002
Type:
Article
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