Effect of emodin on proliferation and differentiation of 3T3-L1 preadipocyte and FAS activity / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1035-1038, 2002.
Article
in English
| WPRIM
| ID: wpr-340391
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of emodin on proliferation and differentiation of 3T3-L1 preadipocyte and the possible mechanism.</p><p><b>METHODS</b>Cell proliferation was determined by MTT spectrophotometry, cell differentiation was determined by Oil Red O staining,and fatty acid synthase (FAS) activity was determined by spectrophotometry.</p><p><b>RESULTS</b>Emodin promoted proliferation of 3T3-L1 preadipocyte at low concentration and inhibited the proliferation at high concentration in a dose-related manner. In contrast, it inhibited cell differentiation into adipocyte at low concentration in a dose-related manner. In vitro emodin inhibited the activity of FAS in a dose-related manner.</p><p><b>CONCLUSIONS</b>The effects of emodin on 3T3-L1 cell's proliferation and differentiation are dose dependent. Emodin inhibits the activity of FAS. Our results suggest that emodin should have a potential to serve as a fat-reducing drug.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Stem Cells
/
Cell Differentiation
/
Cell Division
/
Emodin
/
3T3 Cells
/
Adipocytes
/
Lipid Metabolism
/
Fatty Acid Synthases
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2002
Type:
Article
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