Effects of rhG-CSF Stimulation in vitro on the Adhesion and Polarization of Human CD4⁺T Lymphocytes / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1702-1706, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-340433
ABSTRACT
The adhesion and polarization of T lymphocytes involved in the adhesive interaction of lymphocyte function-associated antigen 1 (LFA-1) with its ligand intercellular adhesion molecule 1 (ICAM-1). This study was aimed to investigate the effects of recombinant human granulocyte colony-stimulating factor (rhG-CSF) stimulation in vitro on the adhesion and polarization of CD4⁺ T cells of healthy human in peripheral blood. The peripheral blood mononuclear cells were collected from 12 healthy volunteers. The CD4⁺ T cells were sorted by miniMACS. The sorted CD4⁺ T cells were incubated with rhG-CSF for 24 h, then the adhesion and polarization of CD4⁺ T cells activated by stroma cell-derived factor -1α (SDF-1α) and ICAM-1 were detected by ELISA and inverted microscope. The results showed that the percentage of adhesion CD4⁺T cells in the experimental group (rhG-CSF acting on the healthy adult volunteers) (61.9 ± 5.9)% was lower than that in the control group (healthy adult volunteers without rhG-CSF stimulation) (68.3 ± 7.3)% (P < 0.05). The percentage of polarized CD4⁺T cells in the experimental group (24.3 ± 4.3)% was also lower than that in control group (47.1 ± 5.1)% (P < 0.05). It is concluded that the adhesion and polarization of CD4⁺T lymphocytes can be inhibited after rhG-CSF stimulation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
In Vitro Techniques
/
Recombinant Proteins
/
Leukocytes, Mononuclear
/
Lymphocyte Activation
/
CD4-Positive T-Lymphocytes
/
Cell Adhesion
/
Cell Movement
/
Granulocyte Colony-Stimulating Factor
/
Lymphocyte Function-Associated Antigen-1
Limits:
Aged
/
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2014
Type:
Article
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