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Mechanisms of cladribine-inducing apoptosis of multiple myeloma RPMI 8226 cells in vitro / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 1644-1648, 2014.
Article in Chinese | WPRIM | ID: wpr-340443
ABSTRACT
This study was purposed to explore the mechanisms of cladribine (2-CdA)-inducing apoptosis of multiple mycloma RPMI 8226 cells. The MTT method was used to determine cell proliferation after being treated with 2-CdA. Apoptosis and cell cycle progression were examined by flow cytometry. Transmission electron microscopy was used to observe ultrastructural changes of RPMI 8226 cells. RT-PCR and Western blot were used to analyze the mRNA and protein expression levels of BCL-2, MCL-2 and caspase-3 respectively. The results showed that the 2-CdA inhibited proliferation of RPMI 8226 cells in time and dose-dependent manner. Typical apoptotic morphological and ultrastructure changes could be observed by electron microscopy. Flow cytometry showed that 2-CdA induced myeloma cell apoptosis and arrested myeloma cells in the G2/M phase. The mRNA expression of BCL-2 and MCL-1 decreased but that of caspase-3 not apparently changed. Western blot results suggested that the change trend of BCL-2 MCL-1 and caspase-3 was the same as result of RT-PCR. It is concluded that 2-CdA exhibits inhibitory effects on RPMI 8226 cells in vitro. Activating the mitochondrial and death receptor pathways of apoptosia may be the potential mechanism, meanwhile, the cell cycle arrest may also play a critical role in apoptosis.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Cell Division / Cladribine / Apoptosis / Proto-Oncogene Proteins c-bcl-2 / Cell Line, Tumor / Cell Proliferation / Caspase 3 / Multiple Myeloma Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Cell Division / Cladribine / Apoptosis / Proto-Oncogene Proteins c-bcl-2 / Cell Line, Tumor / Cell Proliferation / Caspase 3 / Multiple Myeloma Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2014 Type: Article