Clinical significance of detecting peripheral T cell subsets in children with leukemia / 中国实验血液学杂志

ABSTRACT

This study was purposed to investigate the changes of peripheral blood T cells in children with acute leukemia at different stages and understand the immune status of children with leukemia. The CD4⁺, CD8⁺, CD4⁺/ CD8⁺ ratio, CD3⁺ and NK cells in 42 children with acute leukemia and 50 cases of normal children (as control group) were determined by flow cytometry at different periods after complete remission. The results showed that the CD3⁺ CD4⁺, CD8⁺ rate and CD4⁺/CD8⁺ ratio in newly diagnosed ALL and AML children were significantly lower than those in control group (P < 0.05). The NK cell count in newly diagnosed children with acute leukemia was significantly lower than that in control group (P < 0.05). Although the NK cell count in ALL and AML children gradually rose at 3, 6, 12 months after complete remission, but it still was statistically different from normal control group (P < 0.05). It is concluded that children with acute leukima have cellular immune disfunction at onset and during treatment, but the cell immune function gradually recovered after complete remission achieved. However, its recovery rate is slow. The results of this study can provided a basis for subsequently use of immunomodulations in leukemia children.