The role of the PI3K/AKT signaling pathway in DADS-induced apoptosis of K562 cells / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 1050-1054, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-340569
ABSTRACT
<p><b>OBJECTIVE</b>To study the role of the PI3K/AKT signaling pathway in the diallyl disulfide (DADS)-induced apoptosis of K562 cells.</p><p><b>METHODS</b>K562 cells in the logarithmic growth phase were treated with 10, 20, 40, or 80 mg/L DADS for 48 hours, then fixed and stained with acridine orange/ethidium bromide (AO/EB), and examined for cellular morphological changes under an inverted microscope. Annexin V-FITC/PI staining was used for determining the apoptotic rates, and Western blot for measuring the expression of AKT, p-AKT, and Caspase-3. Two control groups, blank and solvent, were used as references.</p><p><b>RESULTS</b>K562 cells treated with DADS for 48 hours exhibited the characteristic morphological features of apoptosis including cell shrinkage, irregular cell shape, and membrane blebbing. AO/EB staining results demonstrated that the number of apoptotic cells with cell shrinkage, pyknotic or bead-like nuclei, chromatin condensation, and orange staining increased with the increasing DADS concentration, and 40 mg/L DADS had the most significant effect. The apoptotic rates of cells treated with 10, 20, 40, and 80 mg/L DADS were all significantly higher than those in the control groups (P<0.05). There were no significant differences in AKT protein expression between the K562 cells treated with different concentrations of DADS; the p-AKT protein expression decreased with the increasing DADS concentration, while the Caspases-3 protein expression increased with the increasing DADS concentration (P<0.05).</p><p><b>CONCLUSIONS</b>DADS induces the apoptosis of K562 cells, probably through inhibiting the protein expression in the PI3K/AKT signaling pathway.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Signal Transduction
/
Apoptosis
/
Phosphatidylinositol 3-Kinases
/
K562 Cells
/
Disulfides
/
Dose-Response Relationship, Drug
/
Allyl Compounds
/
Proto-Oncogene Proteins c-akt
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2016
Type:
Article
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