Establishment of a tight tetracycline-controlled HCV-C double transgenic mouse model / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1530-1533, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-340786
ABSTRACT
<p><b>OBJECTIVE</b>To develop a tight tetracycline-controlled HCV-C double transgenic mouse model.</p><p><b>METHODS</b>By crossbreeding of ApoE-rtTA-tTS transgenic mice with TRE-HCV-C transgenic mice, the double transgenic mice were produced in the F1 generation. The presence of HCV-C and tTS gene in the F1 generation was confirmed by PCR, followed by further identification and quantification of the transgene using Southern blot hybridization. The expression of HCV-C in the liver of the mouse model was detected immunohistochemically.</p><p><b>RESULTS AND CONCLUSION</b>Two transgenic mice were obtained, which contained ApoE-rtTA-tTS and TRE-HCV-C genes in the genome. Five founders contained HCV-C gene as confirmed by PCR and Southern blot hybridization. The tight tetracycline-controlled system may facilitate further study of HCV-C gene expression and gene therapy of hepatic cellular carcinoma.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Apolipoproteins E
/
Pharmacology
/
Tetracycline
/
Breeding
/
Mice, Transgenic
/
Gene Expression Regulation, Viral
/
Trans-Activators
/
Viral Core Proteins
/
Blotting, Southern
/
Polymerase Chain Reaction
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2008
Type:
Article
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