In vitro induced and expanded Epstein Barr virus-specific cytotoxic T lymphocytes can specifically kill nasopharyngeal carcinoma cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 1431-1433, 2008.
Article
in Zh
| WPRIM
| ID: wpr-340802
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for efficient induction and expansion of Epstein Barr virus (EBV)-specific cytotoxic T lymphocytes (CTL) in vitro and evaluate the possibility of using this strategy for treatment of nasopharyngeal carcinoma (NPC).</p><p><b>METHODS</b>EBV-transformed B lymphoblastoid cells (BLCLs) were used as the antigen stimuli and antigen-presenting cells. EBV-specific CTL was induced by co-culture of the autologous peripheral blood mononuclear cells (PBMCs) and the irradiated BLCLs, and expanded with a cocktail method consisting of OKT-3, irradiated homologous PBMC, and IL-2. The specific activity of the CTL against the NPC cells was measured with MTT assay.</p><p><b>RESULTS</b>EBV-specific CTL was successfully induced and expanded by 600 folds. The killing efficiency of the CTL was 76% for autologous BLCLs, 13% for homologous BLCLs, 51% for autologous NPC cells, and 27% for homologous CNE cell line, and after expansion, the corresponding killing efficiencies were 63%, 25%, 49%, and 33%, respectively. The non-specific killing only slightly increased after the expansion.</p><p><b>CONCLUSION</b>EBV-specific CTL can be successfully induced and expanded in vitro for specific killing of autologous NPC cells, suggesting the potential of this strategy in the treatment of NPC.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Therapeutics
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Virology
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Tumor Cells, Cultured
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B-Lymphocytes
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T-Lymphocytes, Cytotoxic
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Cells, Cultured
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Nasopharyngeal Neoplasms
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Immunotherapy, Adoptive
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Herpesvirus 4, Human
Limits:
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2008
Type:
Article