Construction and expression of somatostatin (S14) and hepatitis B surface antigen gene in yeast Pichia pastoris / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 483-486, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-340987
ABSTRACT
To construct the ss/HBsAg protein gene-engineering vaccine for developing the diagnosis and cure tumors in clinical medicine and promoting the growth in animal husbandry production. A pair of primers were designed according separately to the sequence of Somatostatin gene(S14) and HBsAg gene. Their gene fragments were separately amplified by using PCR and cloned, following sequencing, the DNA fragments were inserted into pBluescript vector. Then the ss/HBsAg chimera was constructed and was cloned into pPICZaA plasmid, and transformed into electroporated Pichia pastoris. High yield protein expression was obtained. Expressed protein was proved with high specificity and it's molecular weigh was about 28 KD identified by SDS-PAGE and Western blot.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pichia
/
Recombinant Fusion Proteins
/
Somatostatin
/
Gene Expression
/
Cloning, Molecular
/
Artificial Gene Fusion
/
Genetic Vectors
/
Genetics
/
Hepatitis B Surface Antigens
/
Metabolism
Type of study:
Prognostic study
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2002
Type:
Article
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