Platelet-derived growth factor-BB inhibited p21(WAF1) expression partially through transforming growth factor-beta signalling system in vascular smooth muscle cell / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 160-165, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-341262
ABSTRACT
<p><b>OBJECTIVE</b>To assess if the modulating effect of platelet-derived growth factor (PDGF)-BB on p21(WAF1) was mediated by upregulating transforming growth factor (TGF)-beta(1) expression in vascular smooth muscle cells (VSMC).</p><p><b>METHODS</b>TGF-beta(1) mRNA and protein expressions were measured by reverse transcription-PCR and ELISA, the protein expressions of p21(WAF1) and the downstream TGF-beta signalling including TGF-beta type I receptor (ALK-5 in VSMC), Smurf2, pSmad2/3, Smad4, Smad7 were detected by Western blot.</p><p><b>RESULTS</b>PDGF-BB significantly upregulated the expressions of TGF-beta(1) at mRNA (0.79-fold) and protein (1.98-fold) levels in VSMC, significantly inhibited the expression of p21(WAF1) (-67 +/- 12)%, and enhanced the expressions of ALK-5, pSmad2/3, Smad4, Smurf2 protein by 1.21-fold, 0.95-fold, 0.69-fold and 2.55-fold respectively, inhibited Smad7 expression (-65 +/- 9)%, these alterations were partially restored by anti-TGF-beta(1) neutralizing antibody.</p><p><b>CONCLUSIONS</b>These findings suggested that PDGF-BB inhibited p21(WAF1) expression in VSMC partially through upregulating TGF-beta(1) expression via PDGF-BB and TGF-beta signalling pathways.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Platelet-Derived Growth Factor
/
Signal Transduction
/
Cell Division
/
Cells
/
Rats, Sprague-Dawley
/
Proto-Oncogene Proteins c-sis
/
Cell Biology
/
Myocytes, Smooth Muscle
/
Cyclin-Dependent Kinase Inhibitor p21
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Cardiology
Year:
2010
Type:
Article
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