Coexpression of hepatitis B virus X gene and hepatitis C virus C gene in HepG2 cells and its effect on the expression of VEGF / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 529-531, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-341317
ABSTRACT
<p><b>OBJECTIVE</b>To establish an experimental model of HCV C-HBV X co-expression protein and explore its effect on the expression of VEGF.</p><p><b>METHODS</b>The HBV X gene was recovered by enzyme excision and inserted into PBK-CMV and PBK-HCVC, and recombinant plasmids PBK-X and PBK-X-C were constructed. The plasmids PBK-CMV, PBK-X, PBK-HCVC and PBK-X-C were transfected into HepG2 cells with liposomes. After being selected by G418, resistant colonies were obtained. Reverse transcription PCR and Western blot were used to show HBV X and HCV core protein expression. VEGF was analyzed using immunohistochemical methods and Western blot.</p><p><b>RESULTS</b>The recombinant plasmid PBK-X-C expressed HBV X and HCV core protein efficiently under the control of the vectors promoter. VEGF and VEGF mRNA of the cells co-expressing HCV C-HBV X proteins were higher than those cells expressing HBV X, HCV C and vector alone.</p><p><b>CONCLUSION</b>HBV X-HCV C co-expression protein can increase the expression of VEGF of HepG2 cells. It suggests that HBV and HCV have a synergic action in the carcinogenesis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transfection
/
Gene Expression
/
Gene Expression Regulation, Viral
/
Trans-Activators
/
Viral Core Proteins
/
Hepacivirus
/
Vascular Endothelial Growth Factor A
/
Hep G2 Cells
/
Genetic Vectors
/
Genetics
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2006
Type:
Article
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