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Overexpression of PEMT2 inhibits the phosphorylation and translocation of PLC gamma 1 / 中华肝脏病杂志
Chinese Journal of Hepatology ; (12): 514-516, 2006.
Article in Zh | WPRIM | ID: wpr-341321
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVES</b>To explore the mechanism of CBRH-7919 cell proliferation inhibition by transfecting phosphatidylethanolamine N-methyltransferase 2 gene (PEMT2).</p><p><b>METHODS</b>The effects of PEMT2 transfection on phosphorylation and translocation from cytosol to plasma membrane of PLC gamma 1 in cells were studied using SDS-PAGE and Western blot techniques. The phosphorylation and activity of c-Met were determined.</p><p><b>RESULTS</b>After transfection of pemt2, the PLC gamma 1 and phosphorylated PLC gamma 1 conjugated with plasma membrane were decreased by 45% and 27% of that of control cells respectively, and the phosphorylated c-Met was decreased to 32% of that of control cells.</p><p><b>CONCLUSION</b>Transfection of phosphatidylethanolamine N-methyltransferase 2 gene can inhibit the phosphorylation and translocation from cytosol to plasma membrane of PLC gamma 1 in cells. At the same time, the autophosphorylation of c-Met was decreased, which suggests that transfection of phosphatidylethanolamine N-methyltransferase 2 gene can downregulate the c-Met/PLC gamma 1 signaling pathway in CBRH-7919 cells.</p>
Subject(s)
Full text: 1 Index: WPRIM Main subject: Phosphorylation / Transfection / Cell Movement / Proto-Oncogene Proteins c-met / Cell Line, Tumor / Cell Proliferation / Phospholipase C gamma / Phosphatidylethanolamine N-Methyltransferase / Genetics / Liver Neoplasms, Experimental Limits: Animals Language: Zh Journal: Chinese Journal of Hepatology Year: 2006 Type: Article
Full text: 1 Index: WPRIM Main subject: Phosphorylation / Transfection / Cell Movement / Proto-Oncogene Proteins c-met / Cell Line, Tumor / Cell Proliferation / Phospholipase C gamma / Phosphatidylethanolamine N-Methyltransferase / Genetics / Liver Neoplasms, Experimental Limits: Animals Language: Zh Journal: Chinese Journal of Hepatology Year: 2006 Type: Article