Hepatitis C virus NS5A protein upregulates survivin gene expression / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 414-417, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-341345
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether the nonstructural protein 5A (NS5A) encoded by the human hepatitis C virus RNA genome could affect the expression of the survivin gene.</p><p><b>METHODS</b>HCV NS5A expression plasmid (pCNS5A) was transfected into HepG2 cells using a lipofectin reagent and the HCV NS5A protein was detected using immuno-histochemistry staining. Survivin mRNA was evaluated by reverse transcriptase polymerase chain reaction (RT-PCR) and the expression of survivin protein was detected using Western blot.</p><p><b>RESULTS</b>HCV NS5A protein was detected in the cytoplasm of the HepG2 cells transfected with pCNS5A, and the expressions of survivin mRNA and survivin protein were both up-regulated in the presence of the HCV NS5A protein.</p><p><b>CONCLUSIONS</b>HCV NS5A protein can stimulate survivin protein expression, and this might be the result of the strengthening of the survivin gene transcription.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
RNA-Dependent RNA Polymerase
/
RNA, Messenger
/
Tumor Cells, Cultured
/
Transfection
/
Viral Nonstructural Proteins
/
Carcinoma, Hepatocellular
/
Reverse Transcriptase Polymerase Chain Reaction
/
Inhibitor of Apoptosis Proteins
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2006
Type:
Article
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