Screening the hALR-interacting protein from the cDNA library of hepatocarcinoma cells and studying its biological functions / 中华肝脏病杂志

ABSTRACT

<p><b>OBJECTIVES</b>To screen the hALR-interacting protein by phage-displayed technique and identify its biological activities.</p><p><b>METHODS</b>The specific phage clones that interacted with target protein hALR from a cDNA library of hepatocarcinoma cells were selected using the T7 phage-displayed technique. The acquired cDNA inserts were sequenced and analyzed by bioinformatic tools. The biological activities of the phage-displayed peptide affecting QGY hepatocarcinoma cells were studied using 3H-TdR method.</p><p><b>RESULTS</b>The cDNA inserts with 212 bp were acquired after 4 rounds of biopanning. They showed 100% homology with citron kinase. The phage-displayed peptide and the peptide combined with hALR affected QGY cells proliferation.</p><p><b>CONCLUSIONS</b>hALR-interacting peptide can be specifically screened by phage-displayed technique. Citron kinase that interacted with hALR potentially plays an important role in the proliferation of hepatocarcinoma cells.</p>