Application of melanocytes and bone marrow mesenchymal stem cells in tissue engineered skin construction / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 402-407, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-341394
ABSTRACT
<p><b>OBJECTIVE</b>To explore the method of constructing tissue-engineered skin using melanocytes and bone marrow mesenchymal stem cells (BMSCs) in vivo.</p><p><b>METHODS</b>Melanocytes were isolated from human foreskin. BMSCs were isolated from human bone marrow. Both of them were co-cultured at a ratio of 110, and then were implanted into the collagen membrane to construct the tissue-engineered skin, which was applied for wound repair in nude mice. The effectiveness of wound repair and the distribution of melanocytes were evaluated by morphological observation, in vivo 4,6-diamidino-2-phenylindole, dihydrochloride (DAPI) fluorescent staining tracing, HE staining, S-100 immunohistochemistry, and transmission electron microscopy.</p><p><b>RESULTS</b>The wounds were satisfactorily repaired among the nude mice. The melanocytes were distributed in the skin with normal structure, as confirmed by DAPI fluorescent staining tracing, HE staining, S-100 immunohistochemistry, and transmission electron microscopy.</p><p><b>CONCLUSION</b>Melanocytes and BMSCs, after proper in vitro culture at an appropriate ratio, can construct the tissue-engineered skin with I type collagen membrane.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Skin
/
Wounds and Injuries
/
Bone Marrow Cells
/
Cells, Cultured
/
Skin, Artificial
/
Coculture Techniques
/
Cell Biology
/
Collagen Type I
/
Tissue Engineering
/
Mesenchymal Stem Cells
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2011
Type:
Article
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