Mechanism governing reversal of multidrug resistance in human breast carcinoma cells by chelerythrine / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 45-50, 2011.
Article
in English
| WPRIM
| ID: wpr-341461
ABSTRACT
<p><b>OBJECTIVE</b>To explore the mechanism governing the reversal of multidrug resistance in human breast carcinoma cells by chelerythrine.</p><p><b>METHODS</b>Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were used to determine the expressions of protein kinase Cα (PKCα) and multidrug resistance-related genes ABCG2, ABCC1, MDR1, and P-glycoprotein (P-gp) in MCF-7Taxol cells after treatment with chelerythrine and phorbol-12-myristate-13-acetate (PMA). Also, the antitumor effect of PMA or chelerythrine and effects of PKCα activator or inhibitor in combination with paclitaxel or adriamycin on multidrug resistance in MCF-7Taxol cells were evaluated by MTT.</p><p><b>RESULTS</b>RT-PCR or Western blot showed that the expressions of MDR1 and P-gp were significantly higher in MCF-7Taxol cells exposed to PMA stimuli (both P0.05).</p><p><b>CONCLUSION</b>PKCα inhibitor chelerythrine can reverse multidrug resistance in breast carcinoma cells by inhibiting the expressions of MDR1 and P-gp expression in vitro.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Breast Neoplasms
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
Drug Resistance, Multiple
/
Drug Resistance, Neoplasm
/
ATP Binding Cassette Transporter, Subfamily B
/
Cell Line, Tumor
/
Benzophenanthridines
/
Metabolism
Limits:
Female
/
Humans
Language:
English
Journal:
Acta Academiae Medicinae Sinicae
Year:
2011
Type:
Article
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