A two-phase culture system for megakaryocyte differentiation of human mobilized peripheral blood CD34+ cells / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 373-378, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-341615
ABSTRACT
In our study, a two-phase culture system was developed to acquire large amount of CD41+ and polyploidy cells. Human mobilized peripheral blood CD34+ (PB CD34+) cells were first cultured in expansion medium (Cocktail or CC100 medium) for 3,4,5 or 6 days, and then cultured in megakaryocytic differentiation medium containing TPO and SCF for additional 7, 8 or 9 days. Cell expansion, morphology, CD41+ cell percentage and DNA content were investigated to evaluate the protocol. The result showed that more CD41+ and polyploidy cells could be obtained following the two-phase culture with Cocktail medium than with CC100. Moreover, with 3 days expansion in Cocktail medium plus 7 days in differentiation medium, the initial CD 34+ cells obtained 16-fold expansion of CD41+ cells and 3-fold expansion of polyploidy cells, such obtained level being significantly higher than that of culturing cells with only one step in TPO or TPO+SCF. We conclude that with the two-phase culture system, PB CD34+ cells can expand and differentiate to more CD41+ and polyploidy cells than those cultured only in accordance to the one-stage culture protocol, so a new and highly efficient megakaryocyte differentiation model for megakaryocyte and platelet related researches is provided already.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Stem Cells
/
Blood
/
Blood Cells
/
Megakaryocytes
/
Cell Differentiation
/
Colony-Stimulating Factors
/
Cell Culture Techniques
/
Antigens, CD34
/
Hematopoietic Stem Cell Mobilization
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2010
Type:
Article
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