Impact of experimental conditions on the results of NMR-based metabononmics study / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 241-244, 2010.
Article
in Zh
| WPRIM
| ID: wpr-341644
Responsible library:
WPRO
ABSTRACT
In the process of preparing and analyzing the metabonomics samples, there are lots of influential factors such as sample individual differences, environmental factors and human factors. Here we report a study on 3 healthy male SD rats of different samples carried out by different operators (n=4). It was a two-way experiment designed to explore the impact factors and lay the foundation for future experiments. The cluster analysis was employed in comparing 12 samples. The results showed that the differences of rats between the sample groups had a certain influence on the metabonomics data, and there was relatively little variability between the 4 operators. That is to say, whilst the sample are rather different from one another, a given sample is generally quite similar to itself in metabonomics study.
Full text:
1
Index:
WPRIM
Main subject:
Blood Chemical Analysis
/
Cluster Analysis
/
Reproducibility of Results
/
Rats, Sprague-Dawley
/
Nuclear Magnetic Resonance, Biomolecular
/
Metabolomics
/
Methods
Limits:
Animals
/
Humans
/
Male
Language:
Zh
Journal:
Journal of Biomedical Engineering
Year:
2010
Type:
Article