A rudimentary study of the acid fibroblast growth factor's plant expression vector construction and transformation tobacco / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 126-131, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-341668
ABSTRACT
Acid fibroblast growth factor (aFGF) has great potential in clinical application, but it is very expensive. In order to reduce the cost of production and to make full use of the merits integrated with plant bioreator, we have explored the aFGF in transgenic Tobacco expression. AFGF gene was inserted into plant expression vector pBI121; the acquired plants contained aFGF gene expression vector pBI121-TOAB-aF. Using Agrobacterium-mediated gene transformation of Tobacco and using transgenic Tobacco containing kanamycin and cephalosporin culture medium, we obtained kanamycin resistant transgenic Tobacco plants. PCR detection, RT-PCR detection and Western blot detection confirmed that foreign genes were successfully expressed in Tobacco. These data could serve as a theoretical foundation on which to use the plant bioreactor for production of aFGF.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Nicotiana
/
Recombinant Proteins
/
Fibroblast Growth Factor 1
/
Plants, Genetically Modified
/
Agrobacterium
/
Genetic Vectors
/
Genetics
/
Metabolism
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2010
Type:
Article
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