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Inhibitory effects of ethyl acetate extract of Huanglian Jiedu decoction on hyphae development of Candida albicans / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 4834-4838, 2014.
Article in Chinese | WPRIM | ID: wpr-341807
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of ethyl acetate extract of Huanglian Jiedu decoction (EAHD) on hyphae development of Candida albicans.</p><p><b>METHOD</b>Inverted microscope, fluorescence microscope, SEM were applied to inspect the Morphological change of C. albicans treated by EAHD at different concentrations. Solid agar plate was utilized to evaluate the colony morphology. Quantitative Real-ime PCR(qRT-PCR) was adopted to observe the expression of hyphae-specific genes such as HWP1, ALS3, UME6, CSH1, SUN41, CaPDE2.</p><p><b>RESULT</b>EAHD with concentration of 312 and 1 250 mg . L-1 could inhibit formation of hyphae and colony morphology. The expression of HWP1, ALS3, UME6, CSH1 were downregulated 4. 13, 3. 64, 2. 46, 2. 75 folds ,while the expression of SUN41 were upregulated 7. 26 folds, CaPDE2 keep unchanged.</p><p><b>CONCLUSION</b>EAHD could inhibit formation of hyphae and colony morphologies of C. albicans through downregulating HWP1, ALS3, UME6 and CSH1.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Candida albicans / Fungal Proteins / Drugs, Chinese Herbal / Down-Regulation / Gene Expression Regulation, Fungal / Biofilms / Reverse Transcriptase Polymerase Chain Reaction / Hyphae / Cell Biology Type of study: Diagnostic study Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Candida albicans / Fungal Proteins / Drugs, Chinese Herbal / Down-Regulation / Gene Expression Regulation, Fungal / Biofilms / Reverse Transcriptase Polymerase Chain Reaction / Hyphae / Cell Biology Type of study: Diagnostic study Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2014 Type: Article