Characterization of human lung cancer cell line A549 transfected with human interferon-gamma gene / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 125-128, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-341926
ABSTRACT
<p><b>OBJECTIVE</b>To establish a human lung cancer cell line expressing human interferon-gamma.</p><p><b>METHODS</b>The full-length gene of human interferon-gamma (IFN-gamma) was introduced into the human lung adenocarcinoma cell line A549 through retroviral vector pLXSN. The established cell line A549-IFN-gamma was tested for expression of MHC class I and class II by flow cytometer (FCM) and tested for expression of IFN-gamma by enzyme-lined immunoadsorbent assay (ELISA ). The tumorigenesis of cell line A549-IFN-gamma was tested on nude mice.</p><p><b>RESULTS</b>A high level of IFN-gamma protein was detected in the culture supernatants of cell line A549-IFN-gamma. The expressions of MHC class I and class II on A549-IFN-gamma cells increased significantly (P<0.01), when compared with parental cell line A549. However, there was no significant difference (P<0.05) between the growth of cell line A549-IFN-gamma and A549. Finally, the tumorigenesis test showed that A549-IFN-gamma had lower tumorigenetic effects than A549.</p><p><b>CONCLUSION</b>The results indicate that introduction of human IFN-gamma gene into cell line A549 could increase its immunogenicity and decrease its tumorigenesis. With the established cell line A549-IFN-gamma, a tumor vaccine for human lung cancer may be developed.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Therapeutics
/
Transfection
/
Genetic Therapy
/
Interferon-gamma
/
Cancer Vaccines
/
Cell Line, Tumor
/
Allergy and Immunology
/
Genetics
/
Lung Neoplasms
Limits:
Animals
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2004
Type:
Article
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