Sequence detection of HBV-DNA P and C region in HIV/HBV superinfection subjects with drug resistance to HAART / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 507-509, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-341965
ABSTRACT
<p><b>OBJECTIVE</b>To further study the resistance of HBV to high activity antiretrovirus therapy(HAART).</p><p><b>METHODS</b>HBV-DNA was quantitatively detected by real-time PCR in 36 HIV/HBV superinfection subjects, C region and P region HBV-DNA in high copies HBV-DNA subjects were detected by routine PCR, PCR products were purified and sequenced and compared with the HBV international Genbank using BLSAT softy ware.</p><p><b>RESULT</b>HBV-DNA was positive in 4 of 36 patients (11.1%) and another 3 had low copies(<10(4)copies/ml), one had a high HBV-DNA copies (10(7)copies/ml). It's HBV-DNA C region sequence had mutation on 2 sites (nt 2412 T/C; nt 2413 T/C) and 1 mutation P region (nt 741 A/G, also YMDD/YVDD) compared with HBV international Genbak reference sequence.</p><p><b>CONCLUSION</b>The HBV resistance to HAART may be related with multiple genetic mutations in the C and P regain of HBV-DNA.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
DNA, Viral
/
Base Sequence
/
HIV Infections
/
Chemistry
/
Hepatitis B virus
/
Antiretroviral Therapy, Highly Active
/
Drug Resistance, Viral
/
Drug Therapy
/
Genetics
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2003
Type:
Article
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