TNF-alpha induced reversal of multidrug resistance in human hepatocellular carcinoma cells / 中华外科杂志
Chinese Journal of Surgery
;
(12): 602-604, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-342113
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the reversal of multidrug resistance in the cell line HepG2/ADM induced by TNF-alpha.</p><p><b>METHODS</b>HepG2/ADM cells were incubated with different concentrations of TNF-alpha (100, 500 and 2500 U/ml) for 72 h. Real-time PCR was performed to compare the mRNA levels of MDR1 with PPAR-alpha in the different concentrations of TNF-alpha treated cells. The Annexin V assay was used to check cell apoptosis induced by 0.5 mg/L adriamycin. Rhodamine 123 efflux assay and MTT assay were used to study P-gp activity and drug resistance in each group, respectively.</p><p><b>RESULTS</b>TNF-alpha could induce down-regulation of MDR1 and up-regulation of PPAR-alpha. Meanwhile, it could enhance cell cytotoxicity and cell apoptosis induced by 0.5 mg/L adriamycin.</p><p><b>CONCLUSIONS</b>TNF-alpha could partially reverse the multidrug resistance of HepG2/ADM cells by down-regulating the expression of MDR1 and up-regulating the expression of PPAR-alpha.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
RNA, Messenger
/
Doxorubicin
/
Tumor Necrosis Factor-alpha
/
Apoptosis
/
Carcinoma, Hepatocellular
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
Drug Resistance, Multiple
/
Drug Resistance, Neoplasm
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2007
Type:
Article
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