Reprogramming of adult human neural stem cells into induced pluripotent stem cells / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1138-1143, 2013.
Article
in English
| WPRIM
| ID: wpr-342223
ABSTRACT
<p><b>BACKGROUND</b>Since an effective method for generating induced pluripotent stem cells (iPSCs) from human neural stem cells (hNSCs) can offer us a promising tool for studying brain diseases, here we reported direct reprogramming of adult hNSCs into iPSCs by retroviral transduction of four defined factors.</p><p><b>METHODS</b>NSCs were successfully isolated and cultured from the hippocampus tissue of epilepsy patients. When combined with four factors (OCT3/4, SOX2, KLF4, and c-MYC), iPSCs colonies were successfully obtained.</p><p><b>RESULTS</b>Morphological characterization and specific genetic expression confirmed that these hNSCs-derived iPSCs showed embryonic stem cells-like properties, which include the ability to differentiate into all three germ layers both in vitro and in vivo.</p><p><b>CONCLUSION</b>Our method would be useful for generating human iPSCs from NSCs and provide an important tool for studying neurological diseases.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Immunohistochemistry
/
Cell Differentiation
/
Cells, Cultured
/
Proto-Oncogene Proteins c-myc
/
Reverse Transcriptase Polymerase Chain Reaction
/
Cell Biology
/
Kruppel-Like Transcription Factors
/
Octamer Transcription Factor-3
/
Cellular Reprogramming
Limits:
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2013
Type:
Article
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