Mutation of lambdapL/pR-cI857 system for production of bacterial ghost in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1423-1430, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-342384
ABSTRACT
Bacterial ghost is intact envelope of Gram-negative bacteria, which is produced by the function of the lysis gene E from bacteriophage PhiX174. The expression of the lysis gene E is usually controlled by the thermosensitive lambdapL/pR-cI857 promoter. In this study, we described a mutation (T --> C) at the ninth nucleotide of the OR2 in the lambdapR promoter of the lambdapL/pR-cI857 system by overlap PCR. The bacteriolytic assay showed that the mutation in the lambdapL/pR-cI857 system enhanced the temperature of repressing the expression of gene E up to 37 degrees C. The lysis efficiency of altered lambdapR promoter in Escherichia coli DH5a and avian pathogenic E. coli DE17 was up to 99.9%. The expanded range of temperature will benefit for the production of bacterial ghost.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Bacteriolysis
/
Viral Proteins
/
Virology
/
DNA, Bacterial
/
Molecular Sequence Data
/
Base Sequence
/
Cell Membrane
/
Gene Expression Regulation
/
Promoter Regions, Genetic
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2012
Type:
Article
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