Application of secretary luciferase labeled orthotopic transplant model of hepatocellular carcinoma to evaluate tumor response to interferon-beta gene therapy / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1236-1244, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-342401
ABSTRACT
To establish an orthotopic transplant mouse model of hepatocellular carcinoma (HCC) labeled with secretary luciferase and to study its response to anti-tumor treatment with interferon-beta gene therapy. We labeled the murine hepatoma Hepal-6 cells with secretary Gaussia princeps luciferase (Gluc), and then injected Gluc labeled Hepal-6 cells intrasplenically in C57BL/6 mice. We monitored blood Glue to evaluate the tumor development and anti-tumor effects of hydrodynamic injection with interferon-beta expressing plasmid. We successfully established the orthotopic mouse model of HCC by intrasplenic injection of Glue labeled Hepal-6 cells. The Glue blood assay could reflect the amount of cancer cells in vivo, tumor progression, as well as anti-tumor effect of interferon-beta gene therapy. In conclusion, Gluc labeled orthotopic transplant mouse model of HCC can ex vivo real-time monitor the tumor development and tumor response to treatments.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Therapeutics
/
Blood
/
Genetic Therapy
/
Treatment Outcome
/
Interferon-beta
/
Genes, Reporter
/
Carcinoma, Hepatocellular
/
Bodily Secretions
/
Cell Line, Tumor
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2012
Type:
Article
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