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Over-expressed MiR-103 promotes porcine adipocyte differentiation / 生物工程学报
Chinese Journal of Biotechnology ; (12): 927-936, 2012.
Article in Chinese | WPRIM | ID: wpr-342428
ABSTRACT
To clarify the function of miR-103 in the differentiation of porcine preadipocyte, we carried out real-time PCR to detect the expression pattern of miR-103 during adipogenesis, and clarified its expression tendency through cell differentiation. Then we used adenovirus that overexpressed miR-103 to infect porcine preadipocyte. Subsequently, mRNA and protein expression of adipogenesis marker--PPARgamma and aP2 was analyzed by real-time PCR and Western blotting. At last, Oil-Red O staining was used to detect lipids accumulation in the 8th day after adipogenic inducement. The expression of miR-103 increased during adipocyte differentiation; compared with the control, the preadipocyte infected by pAd-miR-103 had an elevated expression level of adipocyte marker gene PPARgamma, aP2, and obvious lipid droplet was seen in the 8th day after adipogenic inducement. These results showed that miR-103 can enhance adipogenesis in primary cultured porcine adipocytes.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Swine / RNA, Messenger / Molecular Sequence Data / Base Sequence / Transfection / Cell Differentiation / Adenoviridae / Adipocytes / Cell Biology / MicroRNAs Limits: Animals Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2012 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Swine / RNA, Messenger / Molecular Sequence Data / Base Sequence / Transfection / Cell Differentiation / Adenoviridae / Adipocytes / Cell Biology / MicroRNAs Limits: Animals Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2012 Type: Article