Colloidal gold immunochromatographic strip for rapid detection of melamine / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 887-898, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-342432
ABSTRACT
To develop a specific, rapid, and convenient immunochromatography assay (ICA) to detect melamine residues in dairy products and feed sample. Colloidal gold particles labeled with purified monoclonal antibody against anti-melamine were used as the detector reagent. The MEL-OVA (the conjugate of melamine and ovalbumin) and goat anti-mouse melamine IgG were blotted on the test and control regions of nitrocellulose membrane. The strip was then assembled with sample pad, absorbing pad, and dorsal shield. The limit of detection (LOD) is 50 microg/L. The test trip was applied to detect melamine in milk, milk powder, and animal feeds, with detection limits of 100 microg/L for milk, 100 ng/g for milk powder, 200 ng/g for feeds. Compared to LC-MS/MS, the ICA could be used to screen a large number of dairy products and feed samples for melamine residue.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Reagent Strips
/
Triazines
/
Food Contamination
/
Chemistry
/
Chromatography, Affinity
/
Sensitivity and Specificity
/
Gold Colloid
/
Dairy Products
/
Milk
/
Methods
Type of study:
Diagnostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2012
Type:
Article
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