Quantification of the curing effects of phenanthridine on yeast prion PSI+ / 生物工程学报
Chinese Journal of Biotechnology
; (12): 737-746, 2012.
Article
in Zh
| WPRIM
| ID: wpr-342445
Responsible library:
WPRO
ABSTRACT
In order to quantify the curing effects of phenanthridine on yeast prion, we introduced semi-denaturing agarose gel electrophoresis and fluorescence recovery after photobleaching techniques to quantify the curing effects of phenanthridine on yeast prion at the protein and cellular levels with the [PSI+] yeast strain expressing GFP-Sup35p (NGMC). The results showed that these two approaches could precisely quantify the curing effects of phenanthridine on [PSI+] cells. After a treatment for 1 through 5 days with phenanthridine, the curing rates of [PSI+] cells were 0%, 0%, 51.7%, 87.5% and 94.4%, respectively. Meanwhile, we quantified the sizes of Sup35p polymers in phenanthridine induced pink phenotype cells. The aggregation status in 1-2 days phenanthridine treated cells were similar to those in [PSI+] cells, while the aggregation status in 3-5 days phenanthridine treated cells were similar to those in [psi(-)] cells.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Phenanthridines
/
Saccharomyces cerevisiae
/
Computer Simulation
/
Prions
/
Peptide Termination Factors
/
Cell Biology
/
Saccharomyces cerevisiae Proteins
/
Genetics
/
Metabolism
Type of study:
Prognostic_studies
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2012
Type:
Article