Single B cell monoclonal antibody technologies and applications / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 651-660, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-342453
ABSTRACT
Monoclonal antibodies (mAbs) contribute a lot to the development of numerous fields in life science as a pivotal tool in modern biological research. Development of the PCR methods and maturation of antibody production have made it possible to generate mAbs from single human B cells by single cell RT-PCR with successional cloning and expression in vitro. Compared to traditional monoclonal antibody technologies, single B cell technologies require relatively fewer cells, which are highly efficient in obtaining specific mAbs in a rapid way with preservation of the natural heavy and light chain pairing. With so many advantages, single B cell technologies have been proved to be an attractive approach for retrieval of naive and antigen-experienced antibody repertoires generated in vivo, design of rationale structure-based vaccine, evaluation and development of basic B cell biology concepts in health and autoimmunity, and prevention of infectious diseases by passive immunization and therapy for disorders. Accordingly, this review introduced recent progresses in the single B cell technologies for generating monoclonal antibodies and applications.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
B-Lymphocytes
/
Immunologic Techniques
/
Cell Biology
/
Allergy and Immunology
/
Genetics
/
Metabolism
/
Antibodies, Monoclonal
/
Antibody Specificity
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2012
Type:
Article
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