Screening of food-grade microorganisms for biotransformation of D-tagatose and cloning and expression of L-arabinose isomerase / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 592-601, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-342458
ABSTRACT
L-Arabinose isomerase (L-AI) is an intracellular enzyme that catalyzes the reversible isomerization of D-galactose and D-tagatose. Given the widespread use of D-tagatose in the food industry, food-grade microorganisms and the derivation of L-AI for the production of D-tagatose is gaining increased attention. In the current study, food-grade strains from different foods that can convert D-galactose to D-tagatose were screened. According to physiological, biochemical, and 16S rDNA gene analyses, the selected strain was found to share 99% identity with Pediococcus pentosaceus, and was named as Pediococcus pentosaceus PC-5. The araA gene encoding L-AI from Pediococcus pentosaceus PC-5 was cloned and overexpressed in E. coli BL21. The yield of D-tagatose using D-galactose as the substrate catalyzed by the crude enzyme in the presence of Mn2+ was found to be 33% at 40 degrees C.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pediococcus
/
Recombinant Proteins
/
Biotransformation
/
Classification
/
Cloning, Molecular
/
Aldose-Ketose Isomerases
/
Escherichia coli
/
Galactose
/
Genetic Vectors
/
Genetics
Type of study:
Diagnostic study
/
Screening study
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2012
Type:
Article
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