Expression and molecular evolution of recombinant acetylcholinesterase for detection of pesticide residues: a review / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 557-564, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-342461
ABSTRACT
Acetylcholinesterase (AChE) plays a key role in the pesticide determination. However, the extraction of AChE from natural materials has the disadvantages of low yield, complex purification and poor stability. Therefore, the preparation of recombinant AChE with high performance becomes the hot topic of researchers in recent years. In this article we summarize the progress in the expression of recombinant AChE and the improvement of its analytical characteristic. Finally, we point out that the directed evolution strategy combined with surface display technology is the future trend on improving recombinant AChE activity.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pichia
/
Acetylcholinesterase
/
Recombinant Proteins
/
Pesticide Residues
/
Chemistry
/
Cholinesterase Inhibitors
/
Baculoviridae
/
Evolution, Molecular
/
Cell Surface Display Techniques
/
Genetic Vectors
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2012
Type:
Article
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