Screening and identification of stable transfectants of mouse soluble B lymphocyte stimulator / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 897-900, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-342585
ABSTRACT
Mouse colon cancer cells CT26 were transfected with constructed plasmid expressing mouse soluble B lymphocyte stimulator (msBlyS). Single cell clones were selected with 100 microg/ml Zeosin and subcloned by serial limiting dilution. Eight resistant transfectants were isolated and expanded, and five of them displayed the desirable msBlyS cDNA band amplified by semi-quantitative RT-PCR assay. Western blot analysis showed that only msBlyS molecules of the expected size were detected in the cell lysates from transfectants. The supernatant of transfectants could costimulate B cell proliferation in standard costimulation assay. Thus we have successfully screened the stable transfectants expressing high levels of msBlyS in CT26 cells, which could be used as cancer vaccines for further anti-tumor immunotherapy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Recombination, Genetic
/
Recombinant Proteins
/
Tumor Cells, Cultured
/
B-Lymphocytes
/
Transfection
/
Tumor Necrosis Factor-alpha
/
Cloning, Molecular
/
Colonic Neoplasms
/
DNA, Complementary
Type of study:
Diagnostic study
/
Prognostic study
/
Screening study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2004
Type:
Article
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