Construction and identification of rat GDNF gene recombinant retroviral vector and gene transfection to NSC / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 642-646, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-342773
ABSTRACT
By genetic recombinant technique, the rat GDNF cDNA was recombinated to the retroviral vector pLXSN. The recombinant plasmid pLXSN-GDNF was verified by digestion with restriction endonucleases and PCR. Then neural stem cells (NSCs) were infected with pLXSN-GDNF. Immunocytochemistry, RT-PCR and western-blot were used to detect the transfection effect. Results showed that GDNF cDNA was cloned into retroviral vector pLXSN correctly, and the pLXSN-GDNF can infect NSCs efficiently. These results provide the possibility for transplantation and gene therapy with GDNF of nervous system diseases and injury.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stem Cells
/
Recombinant Proteins
/
Genetic Therapy
/
Adenoviridae
/
Cloning, Molecular
/
Gene Transfer Techniques
/
DNA, Complementary
/
Cell Biology
/
Glial Cell Line-Derived Neurotrophic Factor
/
Genetic Vectors
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2008
Type:
Article
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