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Cloning and characterization of the chloramphenicol resistant gene of Serratia marcescens strain KMR-3 / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1016-1021, 2008.
Article in Chinese | WPRIM | ID: wpr-342800
ABSTRACT
Chloramphenicol-resistant gene was cloned and analyzed by constructing genomic DNA library of Serratia marcescens KMR-3. It showed that cloned chloramphenicol-resistant gene encoded a protein product of 397 amino acids. The protein belonged to PRK10473 protein, and it showed 92% similarity to drug resistance transporter, Bcr/CflA subfamily of Serratia proteamaculans 568. Regulation elements including promoter, terminator, Shine-Dalgarno (SD) sequence and transcription start site also were identified.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Serratia marcescens / Chloramphenicol Resistance / Molecular Sequence Data / Base Sequence / Amino Acid Sequence / Classification / Cloning, Molecular / Genetics Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2008 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Serratia marcescens / Chloramphenicol Resistance / Molecular Sequence Data / Base Sequence / Amino Acid Sequence / Classification / Cloning, Molecular / Genetics Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2008 Type: Article