Global expression profiling of Saccharomyces cerevisiae: metabolic remodeling in post-log phase / 生物工程学报
Chinese Journal of Biotechnology
; (12): 962-967, 2008.
Article
in Zh
| WPRIM
| ID: wpr-342808
Responsible library:
WPRO
ABSTRACT
For the purpose of revealing the mechanism of the reduction of yeasts ethanol production rate after entrance of post-log phase, we used microarray to study expression profiles of the yeast Saccharomyces cerevisiae during the transition from mid-log growth phase to post-log growth. The results demonstrate that the global pattern of gene expression is very stable during the mid-log phase. However, a dramatic metabolic remodeling was found when the yeast entries post-log phase, during which many of amino acid synthesis and metabolism related genes are up-regulated, moreover, ion transport, energy generation and storage related genes are also up regulated during this phase, while a large number of genes involved in transposition and DNA recombination are repressed. Central metabolic pathways also engage in metabolic remodeling, within which the genes involved in succinate and a-ketoglutarate synthesis pathways are up regulated, accordance with those of amino acid synthesis and metabolism. These results demonstrate that the increasing demand for amino acids in post-log phase lead to a metabolic transition into TCA cycle and glyoxylate cycle, which subsequently reduce the ethanol production rate. This suggests a global insight into the process of yeast ethanol fermentation.
Full text:
1
Index:
WPRIM
Main subject:
Saccharomyces cerevisiae
/
Gene Expression Regulation, Fungal
/
Succinic Acid
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Oligonucleotide Array Sequence Analysis
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Gene Expression Profiling
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Ethanol
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Genetics
/
Amino Acids
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Ketoglutaric Acids
/
Metabolism
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2008
Type:
Article