High-level production of alkaline polygalacturonate lyase in recombinant Pichia pastoris / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 635-639, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-342858
ABSTRACT
In order to increase the production of alkaline polygalacturonate lyase (PGL) by recombinant Pichia pastoris GS115, the effect of cell and methanol concentration on the PGL production was carefully investigated by single factor experiment. The optimum conditions were listed as follows the cell concentration 122 g/L, the methanol concentration 20 g/L, and the ratio of methanol and cell concentration 0.16-0.20 g/g (methanol/cell). With the glycerol and methanol feeding strategies, the ratio of methanol and cell concentration could be controlled at the range of 0.171 to 0.195 g/g. And the highest PGL activity (430 u/mL) and highest PGL productivity (4.34 u/mL/h) were achieved.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pichia
/
Polysaccharide-Lyases
/
Recombination, Genetic
/
Recombinant Proteins
/
Chemistry
/
Methanol
/
Alkalies
/
Fermentation
/
Genetic Vectors
/
Genetics
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2008
Type:
Article
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