Research on the orientedly immobilized urease via concanavalin A / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 617-621, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-342861
ABSTRACT
Concanavalin A (ConA) is immobilized on a pre-activated chitosan microspheres, and then oriented immobilization of urease is carried out based on the strong interaction between ConA and glycoprotein. The optimum immobilization conditions are as follows glutaraldehyde concentration is 3.5%, ConA concentration 1 mg/mL, ConA pH 7.0 and urease concentration 0.4 mg/mL. For orientedly immobilized urease, the highest activity was allowed at pH 5.0-6.0 and temperature 77 degrees C, and the Michaelis constant (Km) was disclosed to be 11.76 mmol/L by Lineweaver-Burk plot. Compared with the free urease and the randomly immobilized urease, the optimum pH of the orientedly immobilized urease becomes smaller and the pH domain wider. Orientedly immobilized urease presents higher temperature resistance, higher affinity to the substrate, and higher stability of operation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Temperature
/
Urease
/
Glycoproteins
/
Chemistry
/
Glutaral
/
Concanavalin A
/
Chitosan
/
Enzymes, Immobilized
/
Hydrogen-Ion Concentration
/
Metabolism
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2008
Type:
Article
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