The anti-inflammatory effect of Indonesian Areca catechu leaf extract in vitro and in vivo
Nutrition Research and Practice
;
: 267-271, 2014.
Article
in English
| WPRIM
| ID: wpr-34308
ABSTRACT
BACKGROUND/OBJECTIVES:
Overproduction of nitric oxide (NO) by the inducible nitric oxide synthase (iNOS) enzyme can cause inflammation. Cyclooxygenase-2 (COX-2) is also involved in the inflammatory response through regulation of nuclear factor-kappa B (NF-kappaB). Areca catechu is one of the known fruit plants of the Palmaceae family. It has been used for a long time as a source of herbal medicine in Indonesia. In this study, we explored the effect of Indonesian Areca catechu leaf ethanol extract (ACE) in lipopolysaccharide (LPS)-induced inflammation and carrageenan-induced paw edema models. Recently, this natural extract has been in the spotlight because of its efficacy and limited or no toxic side effects. However, the mechanism underlying its anti-inflammatory effect remains to be elucidated. MATERIALS/METHODS:
We measured NO production by using the Griess reagent, and determined the expression levels of inflammation-related proteins, such as iNOS, COX2, and NF-kappaB, by western blot. To confirm the effect of ACE in vivo, we used the carrageenan-induced paw edema model.RESULTS:
Compared to untreated cells, LPS-stimulated RAW 264.7 cells treated with ACE showed reduced NO generation and reduced iNOS and COX-2 expression. We found that the acute inflammatory response was significantly reduced by ACE in the carrageenan-induced paw edema model.CONCLUSION:
Taken together, these results suggest that ACE can inhibit inflammation and modulate NO generation via downregulation of iNOS levels and NF-kappaB signaling in vitro and in vivo. ACE may have a potential medical benefit as an anti-inflammation agent.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Areca
/
Carrageenan
/
Down-Regulation
/
Blotting, Western
/
NF-kappa B
/
Herbal Medicine
/
Ethanol
/
Edema
/
Cyclooxygenase 2
/
Nitric Oxide Synthase Type II
Type of study:
Prognostic study
Limits:
Humans
Country/Region as subject:
Asia
Language:
English
Journal:
Nutrition Research and Practice
Year:
2014
Type:
Article
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