Preparation and identification of monoclonal antibody against PNAS-2 protein / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1269-1272, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-343304
ABSTRACT
This study was purposed to prepare and primarily identify the specific monoclonal antibodies (McAbs) against the apoptosis related protein PNAS-2 so as to provide the essential tool for study of PNAS-2 function. The McAbs against PNAS-2 were prepared via the immunization of mice, cell fusion and cloning using synthetic peptide of PNAS-2 as immunogen; the specificity, titer and subtype of McAb were detected by Western blot, ELISA and immunofluorescence. The results showed that the stable hybridoma cell line S-31-7 producing McAbs against PNAS-2 protein was successfully obtained. The immunoglobulin of the McAb was identified to be IGg1lambda. The titer of ascetic fluid fled McAb were 18,000. A single specific band with 28 kD was shown in Western blot test, and the antigen recognized was present in cell cytoplasm by immunofluorescence. In conclusion, the obtained McAb against PNAS-2 displays strong specificity and high titer, which may be applied to the advanced research on PNAS-2 protein.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Allergy and Immunology
/
Apoptosis Regulatory Proteins
/
Mice, Inbred BALB C
/
Antibodies, Monoclonal
/
Antibody Specificity
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2009
Type:
Article
Similar
MEDLINE
...
LILACS
LIS