Mechanism of C-reactive protein on proliferation of multiple myeloma U266 cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1242-1245, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-343310
ABSTRACT
This study was purposed to investigate the mechanism of C-reactive protein (CRP) on proliferation of U266 cells. The human multiple myeloma cell line U266 was incubated with human CRP (0, 5, 10, 20 mg/L) for 24 hours, then the proliferation level of U266 cells was detected by using blood analyser. The mRNA expressions of survivin and HSP90alpha were examined by RT-PCR. The results showed that the proliferation ratio was increased, as compared with the control group (p<0.05); furthermore, the mRNA levels of survivin and HSP90alpha were up-regulated in proportion to the increased CRP concentrations. There was significant correlation between expression of survivin and HSP90alpha (r=0.737, p<0.0001) in incubated cells. It is concluded that CRP can stimulate the proliferation of MM cells directly by up-regulating the expression of survivin and HSP90alpha in MM cells. CRP can be regarded as a potential target for MM treatment.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
C-Reactive Protein
/
RNA, Messenger
/
Apoptosis
/
HSP90 Heat-Shock Proteins
/
Cell Line, Tumor
/
Cell Proliferation
/
Inhibitor of Apoptosis Proteins
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2009
Type:
Article
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